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The topic of the essay must lie clearly within Biology and not lean into other subjects. So what does this mean? Biology is the study of life,so the essay must deal with living organisms. Biochemical topics can be pursued but must be focussed on the effects on the living thing not the chemical aspects. For example, a study of enzymatic kinematics falls under chemistry but the factors that affect enzymes and how this impacts on the ecological niche of an organism becomes a Biology topic. An Essay which deals with how organisms learn to negotiate a maze is more likely to be considered as Psychology but if the behaviours are related to the survival of the organism then this falls towards Biology.